User:Fermenter User/Notebook/HANC A4 F4

From OpenWetWare

Jump to: navigation, search
Customize your entry pages

HAND A4 (F4)

Contents

Day -7 (Nov 5 Wed)

   <Sung-Hye>            11/5/2008 12:00  Start Steam Generator.  30 psi maintained.


Day -6 (Nov 6 Thu)

   <Sung-Hye>            11/6/2008 12:13  Calibrate pH probe
   <Sung-Hye>            11/6/2008 12:30  Add Media ( (NH4)2SO4 46.25g, K2HPO4 347g, KH2PO4 149.5g)
                                          pH adjust to 7.0
                                          Refill water to 50-L
                         11/6/2008 12:45  Steam Generator Malfunction!!
                                          Plant Ops called.
   

                         Fermentation Ceased until Steam Generator is Fixed!!
   


Day -5 (Nov 7 Fri)

   <Plant Ops>           11/7/2008 (14:00)  Peter Vickers came by and Opened main valve of steam generator which was closed. 

<Sung-Hye> 11/7/2008 (14:08) Sterilization Started Successfully!

11/7/2008 (15:08) Sterilization completed and Vessel is cooled down to 20C 11/7/2008 (15:08) Purge vessel with 50L/min Air

Day -4 (Nov 8 Sat)

Day -3 (Nov 9 Sun)

Day -2 (Nov 10 Mon)

Day -1 (Nov 11 Tue, Remembrance Day)

Day 0 (Nov 12 Wed)

   <Sung-Hye>            11/12/2008  (11:00)  Set temperature of vessel to set point (37C)                          
                         11/12/2008  (12:20)  Add other media components:
                                                  50 mM MgSO4 500 ml
                                                  2M Arginine 500 ml
                                                  10 mM FeSO4 50 ml
                                                  No Antifoam added
                         11/12/2008  (12:30)  Calibrate dO2 probe
   <Minjeet>             11/12/2008  (14:00)  Bring cells to fermenter room
   <Sung-Hye>            11/12/2008  (14:00)  Inoculate cells into fermenter Fermentation time 0:00      
                                              Increase rpm to 300, Decrease Air flow to 10L/min          
                         11/12/2008  (14:30)  Add 10 ml of Antifoam

Day 1 (Nov 13 Thu)

   <Sung-Hye>            11/12/2008  (12:00)  Harvest 70-L fermenter using CEPA Z-41 continuous centrifuge
   <Minjeet>             11/12/2008  (13:00)  Come to pick up cell pellet



Recent changes


Personal tools