User:Fermenter User/Notebook/BROM H1

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Background color codes:
Pastel Green: Major change of setup this time
yellow: Sung-Hye's correction or question to user

Letter color codes:
Black: Basic comment
Red: Emergency Information (Power shutdown, building access, Manager's absence etc)
Blue: Fermentation Setup information
Green: Discussion
Orange: Fermentation hrs

BROM H1 (F6)


Day -1 (Feb 2 Mon):

   <Jadwiga>            2/2/2009 (15:00) Bring media to fermenter room
<Sung-Hye> 2/2/2009 (15:00) Assemble bioreactor 2/2/2009 (15:20) Add 10 ml of Antifoam. 2/2/2009 (15:30) Start Sterilization (Liq 45 min) 2/2/2009 (16:00) Cooling of bioreactor (Temp setup) 1 L/min air, 250 rpm


Day 0 (Feb 3 Tue):

   
    Sung-Hye: CHBE Class Auditing 9:30 - 11:00
   
                       2/3/2009 (8:25) Start Fermentation Software for Fermenter #6
                        2/3/2009 (8:30) Connect Acid (500 ml) and Base (400 ml), prime pumps
                                              Start pH control                   
                        2/3/2009 (8:30) Calibrate dO2 probe with 250 rpm, O2 purge for 30 min
                        2/3/2009 (8:30) Set dO2
                        2/3/2009 (8:30) Completion of bioreactor set up: Ready for inoculation

-----------------------------------------------------------------------------------------------------

<Jadwiga> 2/3/2009 (9:30) Inoculation: inoculation volume (+ 600 ml) Fermentation 0:00 Increase rpm to 400

<Jadwiga> 2/3/2009 (9:30) Sampling Fermentation 0:00 Cell weight ( mg), Activity ( %, x100), Activity ( %, x1000) <Sung-Hye> 2/3/2009 (16:00) Increase rpm to 600 <Jadwiga> 2/3/2009 (00:00) Sampling Fermentation 0:00 Cell weight ( mg), Activity (%, x100), Activity ( %, x1000)

Day 1 (Feb 4 Wed):

  5 am - 9:15 am : O2 tanks ran out 
  <Sung-Hye>            2/4/2009 (9:15) Fermentation 23:45
                                           Open O2 tank
                                           Acid (500 ml) Base (170 ml)
  <Jadwiga>             2/4/2009 (9:30) Sampling  Fermentation 24:00
                                           Cell weight ( mg), Activity ( %, x100), Activity ( %, x1000)
<Jadwiga> 2/4/2009 (00:00) Sampling Fermentation 00:00 Cell weight ( mg), Activity ( %, x100), Activity ( %, x1000)

Day 2 (Feb 5 Thu):

  <Jadwiga>             2/5/2009 (9:30) Sampling  Fermentation 36:00
                                           Cell weight ( mg), Activity ( %, x100), Activity ( %, x1000)
<Sung-Hye> 2/5/2009 (11:30) Fermentation 00:00 Harvest Acid (390 ml) Base (100 ml)






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