User:Fermenter User/Notebook/BROM B11

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Background color codes:
Pastel Green: Major change of setup this time
yellow: Sung-Hye's correction or question to user

Letter color codes:
Black: Basic comment
Red: Emergency Information (Power shutdown, building access, Manager's absence etc)
Blue: Fermentation Setup information
Green: Discussion
Orange: Fermentation hrs

BROM B11


Day 0 (Nov 10 Tue):

   <Sung-Hye>            11/10/2009 12:30 Assemble bioreactor 
   <Vincent>             11/10/2009 12:30 Bring media to fermenter room
   <Sung-Hye>            11/10/2009 13:00 Add 5 ml of (Sterilized) Antifoam
                         11/10/2009 13:25 Start Sterilization (Liq 45 min)
                         11/10/2009 15:18 Cooling of bioreactor (Temp setup)
                         11/10/2009 15:19 Start Fermentation Software for Fermenter #6 Record 0:00  
                         11/10/2009 15:20 Purge with 1.0 L/min O2 
                         11/10/2009 15:22 Connect Acid (500 ml) and Base (400 ml), prime pumps
                         11/10/2009 15:22 Start pH control
                         11/10/2009 15:27 Calibrate dO2 probe with 350 rpm, Air purge for 30 min                    
                         11/10/2009 16:00 Set dO2
  -------------------------------------------------------------------------------------------------------
   <Vincent>             11/10/2009 24:00 Inoculation: (240 ml) Fermentation 0:00


Day 2 (Nov 12 Thu):

   <Vincent>             11/12/2009 (12:00) Sampling Fermentation 36:00
                                          F6: Cell weight (112 mg/ml), Activity ( 91, x100), Activity ( 55, x1000)
   <Vincent>             11/12/2009 (18:00) Sampling Fermentation 42:00
                                          F6: Cell weight (110mg/ml), Activity ( 88, x100), Activity ( 51, x1000)
   <Vincent>             11/12/2009 (18:00)Fermentation 42:00
                                          Harvest

Day 3 (Nov 13 Fri):

                         11/13/2009 (09:00)Fermentation ??:00
                                          F6: Acid (400 ml) Base (190 ml)





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