User:Eric M. Walters/Notebook/Spring 2012/2012/06/25

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Cyanobacterial acrylate production Main project page
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Screening 10 mM AA acsA::BC

  • I got my outside flanking primers, time to screen transformants for purity of BC vs acsA
  • Colony PCR (with 50 degree annealing, 3:50 extension, 35 cycles) gel:
    • Image:Pflegerlab 2012-06-25 17hr 12min acsA;;BC colony PCR (1 of 2).jpg
    • Image:Pflegerlab 2012-06-25 17hr 12min acsA;;BC colony PCR (2 of 2).jpg

Growth on 10 mM AA

  • 1: +
  • 2: ++
  • 3: ++
  • 4: +++
  • 5: +++
  • 6: ++++
  • 7: +++
  • 8: +++
  • 9: +
  • 10: +++++
  • 11: ++
  • 12: +++
  • 13: +
  • 14: ++
  • 15: ++
  • 16: +++
  • 17: ++
  • 18: ++
  • 19: ++
  • 20: ++
  • 21: +++
  • 22: ++
  • 23: ++
  • 24: +++
  • 25: ++++
  • 26: ++
  • 27: +++++
  • 28: +++



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