User:Eric M. Walters/Notebook/Spring 2012/2012/06/08

Cyanobacterial acrylate production	Main project page Previous entry      Next entry
Yet another colony PCR on the acsA::barcode Each 10 uL reaction: 5 uL GoTaq 2x MM 4 uL Nuclease free water .5 uL fresh acsA-UpFv1 primer .5 uL fresh acsA-DnRv1 primer 50 degrees annealing, 3 min extension (acsA is 2kb), 30 cycles so I don't drown out the deletion if it's less prevalent 1-9 are actually: 1, 2, 3, 5, 6, 8, 9, 10, 12