User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/11/30

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Objective

To compare the the 81.9 and 163.8 molar ratios for nanoparticle synthesis and attempt to produce purple fibers at the 69.5 ratio and a purple solutions at the 161 ratio.

Description

81.9 Molar Ratio

Two 81.9 molar ratio reaction mixtures were prepared by mixing 100 uL of 17.7 uM BSA, 50 uL of 2.9 mM HAuCl4. and 850 uL water.

Tube A was left in the heat block set to 80°C for 3 hours. Tube B was placed in the heat block and was taken out for ten minutes every half an hour.


163.8 Molar Ratio

Two 163.8 molar ratio reaction mixtures were prepared by mixing 100 uL of 17.7 uM BSA, 100 uL of 2.9 mM HAuCl4. and 800 uL water.

Tube C was left in the heat block set to 80°C for 3 hours. Tube D was placed in the heat block and was taken out for ten minutes every half an hour.


Data

Image:T=30min.jpg

30 minutes into the reaction, tube B was a light pink color, while tube D had formed a purple clump. Tube A was a light purple-pink color. Tube C appeared for be a purple solution but upon closer inspection, tiny fragments could be seen.

Image:T=90_min_(all_epi_tubes).jpg

This image shows the tubes at the end of the three hour reaction period. The 81.9 molar ratio reaction mixtures all formed purple solutions, while the 163.8 molar ratio reactions all formed purple fibers.

Notes

This area is for any observations or conclusions that you would like to note.


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