User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/10/04

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Objective

To determine the sensitivity of a fluorimeter by using standard concentrations of RU(bpy)(phen) in acetonitrile

Description

  1. 3 mL standards of Ru(bpy)(phen) in acetonitrile were prepared with concentrations ranging from 0 to 35 μM
  2. The solutions were placed in the fluorimeter and the intensities were determined.


Data

The following concentrations of Ru(bpy)(phen) and acetonitrile were used to prepare the standards. Image:October_4_Ru(bpy)(Phen)_concentrations.png


The spectrum of intensities was graphed for each concentration. The intensities recorded by the fluorimeter were corrected with the intensities for pure acetonitrile.

Image:October_4_Ru(bpy)(phen)_standard_intensities.png

Excel was then used to determine the integrated intensities of each standard.

Image:October_5_Integrated_Intensities.png


Image:October_5_Integrated_Intensities_vs._concentration.png


Notes

The color of the Ru(bpy)(phen)solutions became increasingly more orange as the concentrations increased.


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