User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/09/14
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To determine the concentration of an unknown protein by performing a Bradford assay.
Grubb's outlier test was performed to determine if the last data point in the plot was an outlier. The calculations determined that the point was not an outlier. The intercept of the linear regression for the plot has a value of 0.1782. Ideally, the intercept should be at the origin and have a value of 0. The linear regression was used to determine the concentration of the unknown MBP sample by using the corrected absorbance of the unknown sample at 550, which was equal to 0.113. The concentration of the 1000X diluted unknown was calculated to be 0.960 mg/mL. The concentration of the undiluted protein sample was equal to 0.960 g/mL or 1.4 x 10 M. The molar absorptivity of the MBP sample in water was graphed against the wavelength.
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