Objective
Our objective for today was to run UV-vis on the Lysozyme-AuNPs and Myoglobin-AuNPs samples that were synthesized last week. Today we will also be synthesizing new lysozyme-AuNPs and also new Hemoglobin-AuNPs solutions
Protocol
- The samples that were being run on the UV-vis were first centrifuged for 30 minutes before each spectra was taken.
- Protein:AuNP solutions, details of how the solutions were prepared can be found here.
- Lysozyme-AuNPs (protein:Au ratio):
- 5:1
- 10:1
- 15:1
- 20:1
- 25:1
- 30:1
- 35:1
- 40:1
- 45:1
- 50:1
- Hemoglobin-AuNPs (protein:Au ratio)
- 30:1
- 50:1
- 70:1
- 90:1
- 110:1
- 130:1
- 150:1
- 170:1
- 190:1
- 210:1
Data
Absorbance Spectra of Lysozyme-AuNPs
Absorbance of Myoglobin-AuNPs
Additional Observations
- Lysozyome-AuNPs: Only the 30:1 ratio exhibited an absorbance peak at 526nm, this solution appeared colored and did not form fibers. The peak and color observations are characteristic of no fiber formation.
- Myoglobin-AuNPs: Only the 15:1 ratio exhibited an absorbance peak at 732nm, this peak represents the optimal concentration for the formation of fibers.
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