User:Elaine Marie Robbins/Notebook/Capstone/2012/07/13

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FCS of Open MB Main project page
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Methods

  1. Make solutions of 200 nM MB open and closed.
    • Open MB solution contains 10x excess of complimentary strand.
  2. Incubate solutions at 75°C for 30 min.
  3. Incubate solutions at room temp for 30 min.
  4. Run fluorescence.
  5. Run FCS.

Data

  • Fluorescence of 200 nM MB with 10x excess primers, scan speed 10 nm/min:
Image:12-07-13 fluorescence of open MB.png
  • Fluorescence of MB with fluorescein comparison:
Image:12-07-13 fluorescence of open MB with fluorescein.png


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