User:Elaine Marie Robbins/Notebook/Capstone/2012/07/10

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Fluorescence of Open MB Main project page
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Methods

  1. Make solutions of open and closed MB with the following concentrations (open w/ 10x excess of compliment), incubated at 37°C for 1 hr:
    • 10 nM
    • 100 pM
    • 10 pM
  2. Take fluorescence data to make sure MB is open.
  3. Repeat, skipping the heating step over time.

Data

  • Fluorescence of open MB with incubation:
Image:12-07-12 fluroescence of open MB with incubation.png


  • Fluorescence of open MB w/o incubation:
Image:12-07-12 fluorescence of open MB no incubation.png
Image:12-07-12 peak corrected open MB intensity over time.png


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