User:Elaine Marie Robbins/Notebook/Capstone/2012/06/18

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Elongation Without AuNP Main project page
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Methods

  1. Add 1 μL of diluted thiol-modified TS primer to 5.6 mL of citrate AuNP solution.
  2. Mix 3 μL TS primer (no AuNP), 1 μL 50x dNTP mix, 16 μL sterile water, 2.5 μL 10x PCR buffer, and 2 μL cell extract in a PCR tube.
    • Cell dilutions used: 400000, 200000, 100000, 50000, 25000.
  3. Incubate in heat block at 30°C for 30 min.
  4. Add 6.10 μL molecular beacon.
  5. Incubate in heat block at 37°C for 1 hr.
  6. Take fluorescence spectra.

Data

  • Fluorescence data of solutions using just the primer instead of primer/AuNP:
Image:12-06-18 fluorescence without AuNP.png
Image:12-06-18 fluorescence without AuNP magnified.png
  • Fluorescence of solutions without AuNP vs. citrate AuNP solutions synthesized on 06/14/12:
Image:12-06-18 fluorescence without AuNP vs citrate AuNP.png

Changes in citrate AuNP fluorescence most likely due to photo-bleaching of MB over the weekend.


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