User:Elaine Marie Robbins/Notebook/Capstone/2012/04/26

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Addition of Molecular Beacon Main project page
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Methods

  1. Add 6.10 μL molecular beacon to solutions from 04/25/12.
  2. Incubate in heat block at 37°C for 1 hr.
  3. Dilute each solution with 571.9 μL 10 mM tris buffer (pH 7.5)
  4. Take emission fluorescence data of samples (excited at 515 nm):
Image:4-26-12 signal intensity vs emission wavelength.png

Data obtained after subtracting out fluorescence of molecular beacon alone.

References

Caifeng Ding, Xiangling Li, Ying Ge, Shusheng Zhang. Fluorescence Detection of Telomerase Activity in Cancer Cells Based on Isothermal Circular Strand-Displacement Polymerization Reaction. Analytical Chemistry 2010, 82, 2850–2855.



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