User:Elaine Marie Robbins/Notebook/Capstone/2012/04/05

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Methods

  1. Repeat elongation procedure from 03/28/12:
    1. Mix 3 μL AuNP/TS primer, 0.5 μL 50x dNTP mix, 16 μL sterile water, 2.5 μL 10x PCR buffer, and 2 μL cell extract in a PCR tube.
    2. Incubate in an oven at 30°C for 30 min.
    3. Store in refrigerator.
  2. Take UV-vis data of samples:
    Image:4-5-12 absorbance vs wavelength.png
  3. Take emission fluorescence data of samples (excited at 515 nm):
    Image:Signal intensity vs emission wavelength 04-05-12.png

References

Yi Xiao, Karen Y. Dane, Takanori Uzawa, Andrew Csordas, Jiangrong Qian, H. Tom Soh, Patrick S. Daugherty, Eric T. Lagally, Alan J. Heeger, and Kevin W. Plaxco. Detection of Telomerase Activity in High Concentration of Cell Lysates Using Primer-Modified Gold Nanoparticles. Journal of the American Chemical Society 2010, 132, 15299-15307.



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