User:Elaine Marie Robbins/Notebook/Capstone/2012/03/12

Determining AuNP Concentration

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Methods Dilute thiol-modified TS primer with 1 mL of 10 mM tris buffer (pH 7.5). Add 1 μL of diluted thiol-modified TS primer to 5.6 mL of AuNP solution synthesized on 02/15/12. Calculations: ρ Au0 = 19.30 g/mL, diameter AuNP = 32.1 nm, MM Au0 = 196.967 g/mol 2 mL Au+3*(1 L/1000 mL)*(3.66 mmol/1 L)*(1 mol/1000 mmol) = 7.32*10-6 mol Au+3 V AuNP = (4/3)*π*(3.21*10-8 m/2)3 = 1.73*10-17 cm3 1.73*10-17 cm3*(19.30 g/1 mL)*(1 mol/196.967 g) = 1.695*10-18 mol Au/AuNP 7.32*10-6 mol Au+3/1.65*10-18 mol Au/AuNP = 4.32*1012 AuNP 4.32*1012 AuNP/20 mL solution = 2.159*1011 AuNP/mL solution 100 nmol DNA * (1 mol/109 nmol)*(6.02*1023 molecules/1 mol) = 6.02*1016 DNA 1 μL DNA * (1 ml/1000 μL)*(6.02*1016 DNA/1 mL)*(1 AuNP/50 DNA)*(1 mL/2.159*1011 AuNP) = 5.6 mL AuNP References Mandeep Singh Bakshi, Harpreet Kaur, Poonam Khullar, Tarlok Singh Banipal, Gurinder Kaur,Narpinder Singh. Protein Films of Bovine Serum Albumen Conjugated Gold Nanoparticles: A Synthetic Route from Bioconjugated Nanoparticles to Biodegradable Protein Films. Journal of Physical Chemistry 2011, 115, 2982-2992.