User:Elaine Marie Robbins/Notebook/Capstone/2012/03/09

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Preparation of Telomerase Extract Main project page
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Methods

  1. Suspend cells in 200 μL ice cold lysis buffer by retropipeting 3 times.
  2. Incubate on ice for 30 min.
  3. Centrifuge at 13200 rpm for 30 min.
  4. Collect supernatant into EP tubes.

References

Piatyszek, M.A., Kim, N.W., Weinrich, S.L., Hiyama, K., Hiyama, E., Wright, W.E., Shay, J.W., Detection of telomerase activity in human cells and tumors by a telomeric repeat amplification protocol (TRAP). Methods Cell Sci. 1995, 17, 1-15.


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