User:Elaine Marie Robbins/Notebook/CHEM-496/2012/04/10

Objective

To determine the ionic strength of 50 mM tris buffer (pH 10.1) on AuNP fibers.

Description

1. Centrifuge fiber solution made on 03/27/12 at 4500 rpm for 45 minutes.
2. Remove solution, leaving only supernatant.
3. Add 5 mL 50 mM tris (pH 10.1).
4. Centrifuge for 10 min at 13200 rpm.
5. Take UV-vis data.

Data

• Graph of absorbance vs wavelength for tris buffer added to AuNP fibers:

Notes