User:Elaine Marie Robbins/Notebook/CHEM-496/2012/03/07

Au/BSA Mole Ratios II

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Objective To determine the effect of the mole ratio of Au/BSA on AuNP synthesis. Description Make solutions with the following Au/BSA mole ratios using 0.1 mL HAuCl4: 132 Au/BSA 134 Au/BSA 136 Au/BSA 138 Au/BSA 140 Au/BSA 142 Au/BSA 144 Au/BSA 146 Au/BSA Dilute to 1 mL with water. Place in oven at 80°C. Leave solutions in oven for 3 hr. Centrifuge solutions at 13200 rpm for 10 min. Take UV-vis data of supernatants. Data Graph of absorbance vs wavelength for each Au/BSA ratio: Between 132 Au/BSA and 134 Au/BSA, AuNP solution stops forming, and protein fibers begin to form. Notes