User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/22

Ionic Strength Effects of Tris

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Objective To determine the effects of ionic strength on AuNP solution and to continue to take UV-vis data over time of 70 Au/BSA solution synthesized on 01/31/11. Description Add 0.1 mL of tris buffer of varying dilutions (below) to 0.9 mL of AuNP. 0.05 mM 0.5 mM 5.0 mM 50 mM 100 mM 200 mM 500 mM 1 M Data Graph of absorbance vs wavelength for AuNP + varying dilutions of tris buffer: Additions of concentrations of tris 50 mM or above causes an increase in absorbance and a small blue shift in peak wavelength. Graph of absorbance vs wavelength over time for the 70 Au/BSA solution synthesized on 01/31/11. Absorbance values continue to increase over time. However, peak wavelength has leveled off. Notes