Objective
To determine the effects of tris buffer (pH 10.1) on nanoparticle solution and protein fiber solution as a function of concentration and time.
Description
- Take UV-vis data of 70 and 166 Au/BSA solutions prepared on 01/31/12.
- Take UV-vis data of 0.9 mL of each AuNP solution + 0.1 mL of the following dilutions of tris (pH 10.1):
- 50 mM
- 5.0 mM
- 0.50 mM
- 0.050 mM
- Continue to take UV-vis data of 0.9 mL 70 Au/BSA ratio + 0.1 mL 50 mM tris buffer solution over time.
- Continue to take UV-vis data of 1 mL 70 Au/BSA ratio solution over time.
Data
- Graph of the absorbance vs wavelength data shift due to change in buffer concentration for 70 Au/BSA ratio solution:
- Graph of the absorbance vs wavelength data shift due to change in buffer concentration for 166 Au/BSA ratio solution:
- Graph of the absorbance vs wavelength data shift due to time elapse for 70 Au/BSA ratio solution:
- Graph of the peak absorbance vs -log(ion strength of tris buffer solution):
- Graph of the peak wavelength vs -log(ion strength of tris buffer solution):
Notes
The only buffer concentration that produced a shift for the 70 Au/BSA ratio solution in the peak absorbance value was the 50 mM Tris buffer solution (pH 10.1).
No change was observed in the 166 Au/BSA ratio solution.
Absorbance values increase as time elapses for the 70 Au/BSA ratio solution, however there is no shift in peak absorbance value.
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