User:Elaine Marie Robbins/Notebook/CHEM-496/2011/11/08

Objective

To purify the proteins expressed in cells frozen on 09/28/11.

Description

1. Make solutions for tomorrow.
   • 1 L 50 mM Tris (pH 9.02)
   • 1 L 50 mM Tris + 1 M NaCl (pH 9.05)
   • 2 batches of 4 L 50 mM Tris (pH 9.00)
2. Filter both 1 L solutions.
3. Defrost cells from 09/28/11 in a water bath.
4. Sonicate cells: 30 s on, 30 s off 3 times.
5. Centrifuge at 17000 rpm for 1.5 hr.
6. Run dialysis overnight on supernatant in the 2 batches of 4L of 50 mM Tris (pH 9).

Data

Notes