User:Elaine Marie Robbins/Notebook/CHEM-496/2011/10/26
AuNP VI, SDS-PAGE II | Main project page Previous entry Next entry |
ObjectiveAuNP VI To synthesize AuNP using the procedure developed here:
http://pubs.acs.org/doi/abs/10.1021/jp110296y
To run an SDS-PAGE to determine whether or not MBP-intein fusion proteins are being expressed cells comparable to the cells frozen on 09/28/11 and to continue the experiment on 10/25/11. DescriptionAuNP VI
DataAuNP VI After 30 min in the oven, yellow fibers began to appear at the surface of the gold-containing solution. At 60 min, a greenish-gold solid began to form near the surface of the solution. The solution containing only BSA and water remained clear throughout the experiment. SDS-PAGE II The gels indicated that the desired protein was present, but it was not pure.
NotesSDS-PAGE II Protein solutions used were from group 2:
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