User:Elaine Marie Robbins/Notebook/CHEM-496/2011/09/28
|AuNP IV, Expression II|| Main project page|
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To synthesize AuNPs using the procedure developed here:
To continue the experiment on 09/27/11 involving culturing bacteria to express inteins.
The absorbance values for the AuNP solution were subtracted from the absorbance values for a blank acetate buffer to create a set of corrected absorbance values. The corrected absorbance values at 550 nm were graphed against time. The graph shows a positive linear relationship after 60 min, indicating that AuNPs were formed in solution.
In addition, a second graph showing corrected absorbance against wavelength was created, to show the absorbance levels across all of the wavelengths measured by the UV-vis. Absorbance data collected at 150 min from 200 nm-300 nm was ignored, because of outside interference with the UV-vis instrument.
A magnified version of this graph shows that absorbance values at around 550 nm increased over time, indicating the creation of AuNPs.
At 150 min from 200 nm-300 nm, there was interference with the UV-vis, so that data was disregarded.