User:Eduardo Vladimir Munoz/Notebook/UNAM Genomics Mexico 2011/2011/09/15

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High Stabilitiy Plasmid Development

Abstract

  • Gel electrophoresis for corroboration of five strains of repC-ter (possible ligation of repC-PCR and B0015)
  • Replating of three strains of the 5:9 P1004 and B0014 ligation


Gel

1. Ladder 500bp
2. DD B0015 in pSB1AK3 E,X st. 3
3. DD repC-PCR E,S
4. SD repC-ter in pSB1AK3 PstI strain 1
5. SD repC-ter in pSB1AK3 PstI strain 2
6. SD repC-ter in pSB1AK3 PstI strain 5
7. SD repC-ter in pSB1AK3 PstI strain 6
8. SD repC-ter in pSB1AK3 PstI strain 9

Strains 1, 5, 6, 9 are False positives.

Replating

LB medium petri dishes with antibiotics Ampicylin and Kanamycin were made. Three strains of the P1004 and B0014 ligation in a 5:9 ratio were replated in the dishes.


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