User:Eduardo Vladimir Munoz/Notebook/UNAM Genomics Mexico 2011/2011/09/12
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High Stabilitiy Plasmid Development
Using the ligations prepared last saturday I transformed competent DH5α cells. I used 5μL of each ligation for 50μL of cells:
Both repC ligations showed colonies possibly containing the plasmid. Plasmid extraction will proceed. No P1004 colonies were observed.