User:Eduardo Vladimir Munoz/Notebook/UNAM Genomics Mexico 2011/2011/09/12

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High Stabilitiy Plasmid Development

Abstract

  • Transformation of repC and P1004 ligations


Transformations

Using the ligations prepared last saturday I transformed competent DH5α cells. I used 5μL of each ligation for 50μL of cells:

  1. repC-PCR with B0015 in pSB1AK3 in a 6:4 ratio
  2. repC-PCR with B0015 in pSB1AK3 in a 7:4 ratio
  3. P1004 with B0014 in pSB1AK3 in a 7:7 ratio
  4. P1004 with B0014 in pSB1AK3 in a 9:5 ratio

Results

Both repC ligations showed colonies possibly containing the plasmid. Plasmid extraction will proceed. No P1004 colonies were observed.


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