User:Dr. Pascal Dowling

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Dr. Pascal Dowling (an artistic interpretation)
Dr. Pascal Dowling (an artistic interpretation)
  • Dr. Pascal Dowling
  • Pharmaceutical Industry
  • Germany


Scientific Education & Training

  • 2010, Post-doctorate research scientist, Pharmacology, Max-Planck Institute for Heart and Lung Research, Bad Nauheim, Germany

• Research Topics: Cardiopulmonary, epithelial, Orphan GPCRs, neuropharmacology, angiogenesis • Clinical Relevance: Cardiovascular disease, COPD, novel brain receptor targets • Supervisor: Professor Dr. Offermanns • Department: Pharmacology • Techniques: BAC-Transgenics, southern blotting, Flexstation-3, standard molecular biology, IHC, ICC, 293 HEK cells, • Grants: Max Planck Society


  • 2009, Ph.D., Neuroscience, Georg-August-University, Göttingen, Germany

• Research topics: Neuroscience, Clinical Neuroscience, Neuropharmacology, Dopamine, Alzheimer’s, Parkinson’s, mitochondrial, movement disorders, iron-deficiency, D3R-/- mice • Clinical Relevance: Restless legs syndrome, Parkinson’s disease, nutritional deprivation • Supervisor: Professor Dr. Paulus, Professor Dr. Liebetanz • Ph.D. Course structure: Purely research based Doctorate, where a 150 page classical thesis was produced and examined along with publications by a 1-2 hour viva and an intellectual Rigour (Rigorosum) on the topics, intracellular signaling mechanisms and central nervous regulation of sleep and pain. • Program: Georg-August University School of Science (GAUSS) PhD program • Department: Clinical Neurophysiology • Start/End: August 2006/March 2009 • Thesis title: “Impact of iron-deficiency upon behaviour and protein expression in the male mouse with implications for Restless Legs Syndrome”. • Techniques: Animal behavioural experiments (grip-test, running-wheel, hot-plate, formalin-test) were implemented and an dietary induced animal model was established. Retro-orbital sinus blood extraction, transcardial perfusion, tissue extraction, preparation and analysis. Immunohistochemistry, both peroxidase and fluorescence techniques. Molecular techniques were performed; these were 1-D/2-D DIGE electrophoresis, MALDI-TOF-MS and Western Blotting both TANK and Semi-Dry. • Collaborators: Max Planck Institute for Experimental Medicine: Dr. Jahn (Proteomics Group) and Dr. Werner (Department of Neurogenetics) Centre for Clinical Research: Professor Dr. Otto (Neurology Group) University Medical Centre: Professor Dr. Stadelmann-Nessler (Department of Neuropathology) • Examiners: Professor Dr. Flügge, Professor Dr. Kessel, Professor Hardeland • Grants: Deutsche Forschungsgemeinschaft (DFG) funded European Graduate College (GK 632) and GSK • Teaching: Involved in mentoring and lecturing medical and PhD students • Published: Yes (Please refer to the attached publication list)


  • 2009, Postgraduate Researcher, Neuropharmacology, Max-Planck-Institute for Biophysical Chemistry, Göttingen, Germany

• Department: The Prof. Peter Gruss (MPI President) Department of Molecular Cell Biology • Research topics: Neuroscience, Neuropharmacology, Parkinson’s disease, human ES cells, transplantation, dopamine, animal-model • Clinical Relevance: Parkinson’s disease • Supervisor: Professor Dr. Mansouri • Techniques: Murine and human spermatogenic stem cell cultures, differentiation, electroporation. ICC, FACS, cloning pGEM T-Easy vector, Southern blotting. Post-implant IHC dopamine neuron and receptor staining. • Collaborators: Professor Dr. Engel, Professor Dr. Paulus • Grants: Deutsche Forschungsgemeinschaft (DFG) and Max-Planck Society


  • 2006, M.Phil., Neuroscience, Cardiff University, Cardiff, UK

• Research topics: Neuroscience, autonomic nervous system, peripheral ganglia, animal model, senescence • Clinical Relevance: Urinary incontinence • Supervisor: Dr. Santer • M.Phil. Course structure: Purely research based Masters, where a 150 page classical thesis was produced and examined along with other relevant material (publications) by a 3-4 hour viva. • Program: Cardiff Biosciences • Department: Neuroscience Group • Start/End: December 2004/October 2005 • Thesis title: “Age Related Changes in Preganglionic and Postganglionic Neurons in the Major Pelvic Ganglion of the Male Rat.” • Techniques: Transmission electron microscopy (TEM), general IHC procedures and quantitative fluorescence microscopy. • Published: Yes (Please refer to the attached publication list)


  • 2004, B.Sc.(Hons), Pharmacology, University of Manchester, Manchester, UK

• Course Topics: Drug Discovery, Drug Disposition, Neuropharmacology, Advanced Neuropharmacology, Bioinformatics, Endocrinology Biostatistics, Brain Structure and function, Drug Design, Ion Channels and Enzymes as Drug Targets, Pharmaceutical Chemistry, and Molecular Toxicology. • Techniques: DH5α bacteria and 293 HEK cells culturing, ELISA, gene cloning, rodent animal behavioural studies, site-directed mutagenesis, transformation of DH5α bacteria, transfection of mutant DNA, patch clamp and radioligand binding assays, high throughput screening and development of novel compounds from GSK and AstraZeneca. • Final year research project titled: “Interaction of epibatidine with the resting state of the foetal muscle nicotinic acetylcholine receptor.” Employed radioligand binding assays and electrophysiological (patch clamp) studies to show the importance of epibatidine selectivity upon certain residues on γ and δ subunits of the muscle nAChRs when in the resting conformation state. • Final year dissertation: Usage of GABAergic compounds as anti-convulsant therapies • Supervisors: Professor Dive, Professor Weston • Grants: Medical Research Council (MRC)


  • 2001, A-Levels: Chemistry, Mathematics, Physics (Truro College, Cornwall, UK)

Industry Interests

  1. Global medical affairs
  2. Global pharmaceutical markets
  3. Drug discovery & development in Pharma/Biotech
  4. Medical marketing
  5. Medical communications
  6. Strategic medical business development
  7. KOL interaction and management
  8. Perceptual mapping
  9. Medical Education - MSL training
  10. HEOR
  11. Real World Evidence
  12. Emerging markets
  13. Data-analysis
  14. CRM and HCP platform designs
  15. Project design and management
  16. Line management
  17. Medical information
  18. Market access
  • Pain
  • Neurodegenerative diseases
  • Cardiology & COPD
  • Dermatology

Research Interests

  1. Neuro- and psycho-pharmacology
  2. Neurodegenerative diseases
  3. Ageing of PNS and CNS, Alzheimer's, Parkinson's, RLS, schizophrenia, memory, pain
  4. Orphan GPCRs
  5. Pre-clinical model design for industry
  6. Stem cells
  7. Addiction

Research Courses & Techniques

• Centre for Clinical Research: 2-D DIGE and 2-D IEF/SDS-PAGE • MPI Heart & Lung: BAC-transgenics, Flexstation-3 • MPI Experimental Medicine: Proteomics MALDI-TOF-MS and genomics • MPI Biophysical Chemistry: Patch-Clamp - Lab of Nobel laureate Professor Erwin Neher • MPI Biophysical Chemistry: Murine and human ES cell culturing, FACS, Southern blotting, transplantation • UMG: Animal behavioural studies, surgical, blood analysis, ELISA, blotting, confocal microscopy • Cardiff University: Transmission Electron Microscopy • Cardiff University: IHC, dissection, microscopy • University of Manchester: Radioligand binding, basic cell culture and molecular techniques • University of Manchester: Personnel Home Office License for Working under the Animals Scientific Procedure Act 1986 1999. Modules 1-5 (including legal and ethical). • AstraZeneca: High throughput screening and development of novel compounds • SPSS, MINITAB and Prism statistics training courses. • MATLAB programming training

Affiliations

  • British Neuroscience Association
  • German Neuroscience Society
  • Max-Planck Society
  • University of Manchester
  • Cardiff University
  • Georg-August University Göttingen
  • Max-Planck Institute for Biophysical Chemistry
  • Max-Planck Institute for Heart & Lung Research

Publications

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  1. Error fetching PMID 21354213: [Paper1]
  2. Error fetching PMID 21209191: [Paper2]
  3. Error fetching PMID 19776188: [Paper3]
  4. Error fetching PMID 18081164: [Paper4]
  5. Error fetching PMID 17516488: [Paper5]
  6. Error fetching PMID 17074444: [Paper6]
  7. Error fetching PMID 16969922: [Paper7]
All Medline abstracts: PubMed HubMed
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