User:Douglas M. Fox/Notebook/AU CHEM-571 F2011 Lab Support/2012/01/31

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Project 4: BSA-AuNP Biosensors Main project page
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Project Description

Electrochemical Response of a Protein Based Electrode Using Bovine Serum Albumin Encapsulated Gold
February 2012 - April 2012
Project performed in CHEM572 class alongside student projects
Modification of glassy carbon electrodes (GCEs) by physical adsorption of BSA-AuNPs
Control experiments include unmodified GCEs, BSA coated GCEs, acid/heat denatured BSA coated GCEs

Project Objectives

Construction of modified GCEs
Electrochemical response of ferricyanide/ferrocyanide redox couple (CV & EIS)
Electrochemical response of a redox co-factor

Today's Tasks

Preparation of HCl stock solution (control experiment)

  • 150 μL 0.948 M HCl diluted to 50 mL (volumetric flask)
  • 2.83 mM HCl
  • sealed with glass stopper & parafilm

Polished glassy carbon electrodes (GCEs)

  • aided by Melissa Novy
  • 2 GCEs will be used
  • used P1200 grit wet SiC paper (15.3 μm), producing dull grey surface
  • switched to P2400 grit wet SiC paper (6.5 μm), producing shiny surface
  • 100 figure 8s (1/4 turn every 20) using cloth pad & 1 μm, 1/4 μm, & 1/10 μm diamond solution
  • rough surface smoothed out, but tiny scratches still visible (see images below)
  • electrode post still needs to be cleaned (corrosion visble on surface)


Notes:
Images taken using ProScope PR (100x lens)
Prepolished surface (left) was very coarse and not removed by 200 strokes on 1 μm diamond dust
Polished surface (right) had minor scratches; diamond dust solution may remove them



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