User:Dhea Patel/Notebook/Phosphorylation/2012/09/12

Phosphorylation Lab

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Objective to make 35mL of saturated ammonium PF6 to make solvent of 2:4:4:3 isopropanol:dioxane:H2O:NHsub>4OH Description Making Saturated Ammonium PF6 Solution 13.5577g of Ammonium PF6 was added to 35mL water in a 50mL centrifuge tube. Making 2:4:4:3 Isopropanol:Dioxane:H2O:NHsub>4OH Solvent 6mL Isopropanol, 12mL p-Dioxane, 12mL water, and 9mL NH4OH were measured out and combined in a 50mL centrifuge tube. (Total volume = 39mL) The solution was tested by running a TCL of ATP in this solvent. The ATP ran in the solvent made. Data Add data and results here... Notes This area is for any observations or conclusions that you would like to note.