User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/11/17

Cell Fate Switch by Synthetic Transcription Factors

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ELISA (test) Continuing Protocol from NAP blocking stage. Protocol here Primary Antibodies:       *FL180 (Santa Cruz Biotech)- Glucagon (Rabbit- concentration 200ug/mL) - diluted 1/50       *ab7842 (ABCAM) - Insulin (Guinea Pig- concentration 200ug/mL) - diluted 1/50 Insulin primary not meant for ELISA? Testing anyway Secondary Antibodies:       *sc-2030 (Santa Cruz Biotech)- anti-Rabbit IgG-HRP (200 ug/0.5mL) - diluted 1/500       *sc-2438 (Santa Cruz Biotech - anti-Guinea pig IgG-HRP (200ug/0.5mL) - diluted 1/500 Pancreatic Cell Culture Cells look near 60% confluence - FBS worked? RT-PCR Dilutions of Target Primers to 1/10 (100uM)