User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/06/25

Cell Fate Switch by Synthetic Transcription Factors

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RT-PCR E004 Standard Procedure: SYBRGreen Assay Target Genes Gene dilution RTPCR number +/- PcTF PDX1 1:10 A1-3 treated PDX1 1:10 A4-7 untreated MAFA 1:10 B1-3 treated MAFA 1:10 B4-7 untreated GLP1R 1:10 C1-3 treated GLP1R 1:10 C4-7 untreated PCSK1 1:10 D1-3 treated PCSK1 1:10 D4-7 untreated IAPP 1:10 E1-3 treated IAPP 1:10 E4-7 untreated KCNQ2 1:10 F1-3 treated KCNQ2 1:10 F4-7 untreated mCh 1:1000 G1-3 treated mCh 1:1000 G4-7 untreated ACTB 1:1000 H1-3 treated ACTB 1:1000 H4-7 untreated nTc --- A,B,C,D,E,F,G,H8-10 --- Results Gene dilution +/- PcTF Mean CP delta CP double delta CP PDX1 1:10 treated 0 0 Invalid PDX1 1:10 untreated 0 0 0 MAFA 1:10 treated 34.440 0.3350 0.3624 MAFA 1:10 untreated 34.936 0.9245 1 GLP1R 1:10 treated 40 7.10E-3 0.2568 GLP1R 1:10 untreated 40 2.76E-2 1 PCSK1 1:10 treated 0 Invalid Invalid PCSK1 1:10 untreated 0 Invalid Invalid IAPP 1:10 treated 38.363 2.21E-2 3.14E-2 IAPP 1:10 untreated 35.333 0.7022 1 KCNQ2 1:10 treated 31.621 2.363 0.5395 KCNQ2 1:10 untreated 32.6919 4.381 1 mCh 1:1000 treated 33.3063 0.7350 Invalid mCh 1:1000 untreated 0 0 Invalid ACTB 1:1000 treated 32.862 --- --- ACTB 1:1000 untreated 34.823 --- ---