User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/09/10

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Remains of the Day

  • Design Oomycete specific COI primer (include some fungal and protist COI sequences to make sure they don't amplify those) - changed my mind because there's no specificity testing with these on environmental samples, whereas Dr. Levesque shared primers with me that were used for 454 sequencing (so I chose ITS instead)
  • Design and order Biotin labelled ITS4 primer for physical TRFLP
  • Plant potato seeds and minitubers in autoclaved sand and in delhi soil - water with giberelellin
  • Extract rhizosphere off of Tilbury tomato roots



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