User:David Benjamin Nyer/Notebook/PcTF breast cancer/2015/11/09

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Summary

Ideal neon transfection conditions for BT-474 and BT-549 cell lines (so far):

BT-474

Pulse voltage Pulse width Pulse no. Transfection Efficiency Cell Recovery (normalized to control)
105030258.86%32.86%
130010343.27%30.24%
95030242.12%38.57%


BT-549

Pulse voltage Pulse width Pulse no. Transfection Efficiency Cell Recovery (normalized to control)
140020135.08%30.67%
120020231.05%26.99%
120030129.48%30.67%


Will use these values to create a list of transfection conditions for 12-well plates tomorrow.


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