Summary
Here are the neon transfection results:
There's a pretty clear correlation between increasing voltage and both increased transfection efficiency AND decreased cell recovery. Trick will be to find the sweet spot between the two extremes...
BT-474 data:
Sample
|
Well no.
|
Pulse voltage
|
Pulse width
|
Pulse no.
|
Transfection Efficiency
|
Live (cells/µL)
|
Dead (cells/µL)
|
Cell Viability
|
Cell Viability (normalized to control)
|
1 |
A1 |
- |
- |
- |
0.10% |
420 |
5 |
98.82% |
100.00%
|
2 |
A2 |
1400 |
20 |
1 |
31.91% |
33 |
7 |
82.50% |
7.86%
|
3 |
A3 |
1500 |
20 |
1 |
52.07% |
12 |
12 |
50.00% |
2.86%
|
4 |
A4 |
1600 |
20 |
1 |
67.65% |
3 |
8 |
27.27% |
0.71%
|
5 |
A5 |
1700 |
20 |
1 |
74.42% |
9 |
16 |
36.00% |
2.14%
|
6 |
A6 |
1100 |
30 |
1 |
27.72% |
95 |
10 |
90.48% |
22.62%
|
7 |
B1 |
1200 |
30 |
1 |
39.80% |
49 |
8 |
85.96% |
11.67%
|
8 |
B2 |
1300 |
30 |
1 |
43.37% |
8 |
6 |
57.14% |
1.90%
|
9 |
B3 |
1400 |
30 |
1 |
71.02% |
18 |
7 |
72.00% |
4.29%
|
10 |
B4 |
1000 |
40 |
1 |
29.46% |
114 |
7 |
94.21% |
27.14%
|
11 |
B5 |
1100 |
40 |
1 |
42.74% |
36 |
7 |
83.72% |
8.57%
|
12 |
B6 |
1200 |
40 |
1 |
68.75% |
43 |
16 |
72.88% |
10.24%
|
13 |
C1 |
1100 |
20 |
2 |
41.21% |
81 |
7 |
92.05% |
19.29%
|
14 |
C2 |
1200 |
20 |
2 |
58.26% |
73 |
9 |
89.02% |
17.38%
|
15 |
C3 |
1300 |
20 |
2 |
77.55% |
20 |
4 |
83.33% |
4.76%
|
16 |
C4 |
1400 |
20 |
2 |
87.93% |
12 |
4 |
75.00% |
2.86%
|
17 |
C5 |
850 |
30 |
2 |
18.07% |
109 |
22 |
83.21% |
25.95%
|
18 |
C6 |
950 |
30 |
2 |
42.12% |
162 |
29 |
84.82% |
38.57%
|
19 |
D1 |
1050 |
30 |
2 |
58.86% |
138 |
14 |
90.79% |
32.86%
|
20 |
D2 |
1150 |
30 |
2 |
71.43% |
4 |
2 |
66.67% |
0.95%
|
21 |
D3 |
1300 |
10 |
3 |
43.27% |
127 |
55 |
69.78% |
30.24%
|
22 |
D4 |
1400 |
10 |
3 |
53.64% |
34 |
16 |
68.00% |
8.10%
|
23 |
D5 |
1500 |
10 |
3 |
68.13% |
52 |
12 |
81.25% |
12.38%
|
24 |
D6 |
1600 |
10 |
3 |
75.87% |
32 |
14 |
69.57% |
7.62%
|
BT-549 data:
Sample
|
Well no.
|
Pulse voltage
|
Pulse width
|
Pulse no.
|
Transfection Efficiency
|
Live (cells/µL)
|
Dead (cells/µL)
|
Cell Viability
|
Cell Viability (normalized to control)
|
1 |
A1 |
- |
- |
- |
0.24% |
163 |
25 |
86.70% |
100.00%
|
2 |
A2 |
1400 |
20 |
1 |
35.08% |
50 |
5 |
90.91% |
30.67%
|
3 |
A3 |
1500 |
20 |
1 |
36.76% |
19 |
3 |
86.36% |
11.66%
|
4 |
A4 |
1600 |
20 |
1 |
43.33% |
18 |
2 |
90.00% |
11.04%
|
5 |
A5 |
1700 |
20 |
1 |
56.67% |
3 |
1 |
75.00% |
1.84%
|
6* |
A6 |
1100 |
30 |
1 |
0.48% |
124 |
45 |
73.37% |
76.07%
|
7 |
B1 |
1200 |
30 |
1 |
29.48% |
50 |
9 |
84.75% |
30.67%
|
8 |
B2 |
1300 |
30 |
1 |
37.29% |
24 |
5 |
82.76% |
14.72%
|
9 |
B3 |
1400 |
30 |
1 |
33.33% |
2 |
1 |
66.67% |
1.23%
|
10 |
B4 |
1000 |
40 |
1 |
19.64% |
62 |
11 |
84.93% |
38.04%
|
11 |
B5 |
1100 |
40 |
1 |
30.50% |
34 |
5 |
87.18% |
20.86%
|
12 |
B6 |
1200 |
40 |
1 |
41.74% |
23 |
3 |
88.46% |
14.11%
|
13 |
C1 |
1100 |
20 |
2 |
23.41% |
55 |
16 |
77.46% |
33.74%
|
14 |
C2 |
1200 |
20 |
2 |
31.05% |
44 |
10 |
81.48% |
26.99%
|
15 |
C3 |
1300 |
20 |
2 |
42.46% |
18 |
3 |
85.71% |
11.04%
|
16 |
C4 |
1400 |
20 |
2 |
44.58% |
8 |
2 |
80.00% |
4.91%
|
17 |
C5 |
850 |
30 |
2 |
12.60% |
89 |
25 |
78.07% |
54.60%
|
18 |
C6 |
950 |
30 |
2 |
26.27% |
57 |
12 |
82.61% |
34.97%
|
19 |
D1 |
1050 |
30 |
2 |
30.96% |
24 |
7 |
77.42% |
14.72%
|
20* |
D2 |
1150 |
30 |
2 |
0.64% |
94 |
38 |
71.21% |
57.67%
|
21 |
D3 |
1300 |
10 |
3 |
22.56% |
39 |
7 |
84.78% |
23.93%
|
22 |
D4 |
1400 |
10 |
3 |
29.59% |
27 |
6 |
81.82% |
16.56%
|
23 |
D5 |
1500 |
10 |
3 |
37.84% |
11 |
5 |
68.75% |
6.75%
|
24 |
D6 |
1600 |
10 |
3 |
50.65% |
8 |
2 |
80.00% |
4.91%
|
|