User:Daniela A. Garcia S./Notebook/Modeling UNAM-Genomics Mexico/2010/08/23

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WetLab: LovTap's promoters

Ligation LovTap + promoters

LovTap (colony_1) was ligated to seven promoters with different strenght: J23_102, J23_105, J23_106, J23_110, J23_114, J23_116, J23_117. The promoters are in the the plasmid J61002[Amp], these were digested with SpeI and PstI. and desphosphorylated. LovTap was digested with XbaI-PstI

Ligation Protocol

  • DNA(LovTap) ..............6μL
  • Plasmid (promoter) .....2μL
  • Buffer 10X T4 Ligase ...2μL
  • Enzyme T4 Ligase .....1μL
  • HPLC ........................9μL

TOTAL 20μL

The mixture were left ON @ 16ºC



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