User:Daniela A. Garcia S./Notebook/Modeling UNAM-Genomics Mexico/2010/08/23
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WetLab: LovTap's promotersLigation LovTap + promotersLovTap (colony_1) was ligated to seven promoters with different strenght: J23_102, J23_105, J23_106, J23_110, J23_114, J23_116, J23_117. The promoters are in the the plasmid J61002[Amp], these were digested with SpeI and PstI. and desphosphorylated. LovTap was digested with XbaI-PstI Ligation Protocol
TOTAL 20μL The mixture were left ON @ 16ºC
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