User:Daniela A. Garcia S./Notebook/Modeling UNAM-Genomics Mexico/2010/08/04

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Wet Lab: OmpC/F promoter OmpC transformation reporter The OmpC tranformation from 03/08/10 was unsuccesful. It was got colonies just for the positive control BBa_I51020. The procedure would be done again, this time with 7μL of BBa_R0082. OmpC transformation 50 μL DH5α for each plasmid 7 μL of BBa_R0082 5 μL plasmid N. 19 1) 20' on ice 2) 1' 42ºC 3) 5' on ice 4) 60' in 1 ml of LB medium at 37ºC 5) 1' at 13000 rpm Two petri boxes were made with LBAm to plaque the transformed cells. 100 μL were added and left overnight at 37ºC.