User:Daniela A. Garcia S./Notebook/Modeling UNAM-Genomics Mexico/2010/07/27

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Wet Lab: OmpF/C promoters characterization

OmpF PCR

PSB3K3

  • PCR positive control
    • FWD prefix 5μM
    • REV sufix 5μM
  • PCR OmpF
    • FWD sufix 5μM
    • REV 4136 5μM

PSB1T3

  • PCR positive control
    • FWD prefix 5μM
    • REV sufix 5μM
  • PCR OmpF
    • FWD sufix 5μM
    • REV 4136 5μM

RTTH HotStart reactions









PCR's Programme

  • 94ºC, 5:00
  • 35x
    • 94ºC, 0:45
    • 55ºC, 0:45
    • 72ºC, 3:00
  • 94ºC, 10:00
  • 4ºC, ...




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