General
From the article: "Kinetics of Light Emission and Oxygen Consumption by Bioluminescent Bacteria"
- Oxygen consumption by luciferase has been evaluated to be 17% of the total respiration (1)
- Luciferase, regulatory enzyme presenting a positive cooperative effect with oxygen. Michaelis constant for luciferase has been evaluated to be in the range of 20 to 65 nM O2.(1)
- O2 concentrations needed to produce half-maximal light emission range between 0.015 and 0.700 microM(4)
- Vibrio fisheri exhibits an intermediate pattern between a tonic and a phasic bacteria.(5)
- The relationship between intensity of light emission and O2 is best observed in three phases: i) initial light O2 uptake rate ocurring at the burst of luminiscence. ii) steady-state conditions for both muninescence and O2 consumption [duration species and substrate-dependent]. iii)Sharp decrease in both parameters.(5)
- Proportion of luciferase pathway to the total oxygen consumption has been evaluated to be 11.7+-2.1%(6)
- FMNH2 has appeared to be mostly free when it is involved in the muninescent system. Luciferase has roved to have a stricly cytoplasmic localization[V.harveyi](8)
- Cyanide at millimolar concentrations could form with free aldehyde an inhibitory complex of luciferase.
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