User:Daniela A. Garcia S./Notebook/Modeling 2011 UNAM Genomics-Mexico/2011/04/04

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Modeling Meeting FBA analysis in E. coli and R. etli We have both S, so it is possible to add the reactions that our heterologous expression would produce. These analysis would help us to determine to what extent the system is affected (shadow cost) Reactions pyruvato ↔ acetyl-CoA + e- (PFOR) 2H+ + 2e- ↔ H2 (HydA) In order to add the new reactions we idenfied the metabolities already present in S. × R. etli E.coli aceyl-CoA accoa(c) accoa[c/e] pyruvate pyr(c) pyr[c/e] H+ h(c) h[c/e] H2 0 0 e- 0 0