User:Daniel-Mario Larco/Notebook/AU Photosynthesis Lab/2014/02/27

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Protein Extraction The Cell Pellet in tris was thawed in warm water for half an hour The cell pellet was centrifuged at 18,000rpm for 2 hours. The supernatant and pellet were separated and placed in the freezer. Urea Preparation A 5M solution of Urea was prepared 30.03g of Urea were dissolved in 100mL Tris Buffer at pH=7.5