User:Daniel-Mario Larco/Notebook/AU Biodesign Lab - 09/03/2013/2014/04/08

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Objective Testing for HRP (UV/Vis) Spin down HRP GNPs and resuspend pellets for second time Work on paper and presentation Tomorrow: Kinetics Procedure Run UV-Vis on HRP conjugate pellets and supernatants AA of samples to ensure that gold is present Calibration of Javier's Notebook used OPD UV/Vis Notes Gamma method #2 did not work Took AA of gamma supernatant No gold, so the gold never attached or it attached and then fell off Differences between this batch and the other batch More gold nanoparticles in this batch than previous This was after second spin This could mean there was more time for them to fall into solution The time difference alone between attachment could show that the particles are not staying attached.