User:Daneil J. Cipriano/Notebook/Single molecule studies of SNARE NSF interactions/2010/05/11
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Today assemble channels made from glass coverslips blocked with PEG5000 and functionalized with NTA.
1.) Flow in 10 mM NiCl2
2.) Wash with PBS
3.) Flow in 100 pM SNARE in PBS with 0.01 mg/ml BSA included. Incubate 30 min.