User:Cosette Brianna Taggart/Notebook/Experimental/2016/10/12

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October 12, 2016 : Fluorescence V

Objective

  1. Collect fluorescence data for lysozyme at pH 8
  2. Make stock solutions for UV-Vis on 10.18.2016

Description

Fluoresence

  1. Lysozyme solution was made according to the measurements found in data below
  2. Lysozyme solution was run through florescence for 3 hours with data taken every 3 minutes

Stock Solutions for 10.18.2016

Solutions were made in glass test tubes, covered with foil, and placed in an oven for 4 hours at 80 C.

Data

SUMMARY DATA AND FIGURES

Summary Data at 15 minute increments
Time(minutes) Maximum Emission Integrated Intensity
0 356 4414.731619
15 423.5 3579.335338
30 420.5 3239.795939
45 425 3513.64021
60 423.5 4017.276769
75 427.5 4580.514548
90 425 5314.338283
105 423 5820.466363
120 428 6125.368295
135 432 6565.757233
150 426 7092.662926
165 425.5 7338.799533
180 426.5 7714.330801

Notes


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