User:Cosette Brianna Taggart/Notebook/Experimental/2016/09/14

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September 14, 2016 : UV-Vis II

Objective

  1. Run UV-Vis on gold nanoparticle solutions prepped 09.13.16

Description

UV-Vis

  1. Solutions prepped on 09.13.16 were collected for use on UV-Vis
  2. Each solution, individually, was added to the cuvette
  3. The cuvette was added to the UV-Vis machine and run from 800nm to 200nm
  4. Once the run was complete the cuvette was cleaned with 2 soap, 2 water, 2 HCl and 2 water flushes. Followed by a thorough drying.

Data

0.125 mM Fructose
pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of Lysozymestock μL of Water
4 500 0 0 274 48 268 3910
5 50 0 0 274 48 268 4360
6 5 0 0 274 48 268 4405
7 0 0 0 274 48 268 4410
8 0 5 0 274 48 268 4405
9 0 50 0 274 48 268 4360
10 0 500 0 274 48 268 3910
11 0 0 5 274 48 268 4405
12 0 0 50 274 48 268 4360


0.25 mM Fructose
pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of Lysozymestock μL of Water
4 500 0 0 274 96 268 3862
5 50 0 0 274 96 268 4312
6 5 0 0 274 96 268 4357
7 0 0 0 274 96 268 4362
8 0 5 0 274 96 268 4357
9 0 50 0 274 96 268 4312
10 0 500 0 274 96 268 3862
11 0 0 5 274 96 268 4357
12 0 0 50 274 96 268 4312









Notes

Samples at pH 11 contained precipitate.


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