User:Christian G Ramos
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Contact Info
- Christian G Ramos
- M Carmo Fonseca Lab
- Intituto de Medicina Molecular JLA
- Faculdade de Medicina da Universidade de Lisboa
- Avenida Professor Egas Moniz . 1649-028 Lisboa . Portugal.
- Email me through OpenWetWare
Education
- 2011, PhD, Instituto Superior Técnico, Universidade Técnica de Lisboa (Molecular Microbiology)
- 2007, MS, Instituto Superior Técnico, Universidade Técnica de Lisboa (Biotechnology)
- 2002, BS, Universidade Lusófona de Humanidades e Tecnologias, Lisboa (Biotechnology Engineering)
Research interests
Co-transcriptional and post-transcriptional regulation by non-coding RNAs. Splicing regulation. RNA-Protein interactions. Circadian Clock regulation. Neuro-degenerative diseases. Cardiovascular disease, hypertension and sleep regulation.
Publications
Books
Ramos CG. Unveiling Novel Virulence Factors from Burkholderia cepacia Complex: Exploitation of the Caenorhabditis elegans infection model, Lambert Academic Publishing, Saarbrücken, Germany 2011, (ISBN: 978-3-8454-2283-1); Ramos CG, Grilo AM, Leitão JH. Small non-coding RNAs in Prokaryotes: Roles in Pathogenesis and Potential Therapeutic Targets. Lambert Academic Publishing, Saarbrücken, Germany, 2010 (ISBN: 978-3-8383-2116-5).
Book chapters
1. Leitão JH., Sousa SA, Ramos CG, Feliciano JR, Grilo AM, da Costa PJP. Experimental and bioinformatics approaches to identify new targets for the rational design of antimicrobials against multiresistant bacteria of the Burkholderia cepacia complex. In Microbial pathogens and strategies for combating them: science, technology and education, A. Méndez-Vilas (Ed.), Vol. 1,l pp 372-383. ISBN: 978-84-939843-9-7.
2. Ramos CG, Leitão JH. "Caenorhabditis elegans as a research tool to unveil bacterial virulence determinants: Lessons from the Burkholderia cepacia complex" (Chapter 5). In: Nematodes: Morphology, Functions and Management Strategies. (Editors: Fausto Boeri and Jordan A. Chung) Nova Science Publishers, Inc. 2012 (ISBN: 978-1-61470-867-4);
3. Ramos CG, Grilo AM, da Costa PJP, Nadais H, Leitão JH, "Extraction and Purification of DNA obtained from UASB reactors sludge samples". In: DNA Binding and DNA Extraction: Methods, Applications and Limitations. (Editors: Chunxu Zhou and Xia Ling) Nova Science Publishers, Inc. 2011 (ISBN: 978-1-62100-022-8);
4. Sousa SA, Ramos CG, Feliciano JR, Leitão JH, “Bacterial type II PMIs: exploitable bifunctional enzymes for biotechnological applications and the rational design of antimicrobials” (Chapter 15) in Biopolymers (Edited by Magdy Elnashar), 2010 published by Sciyo (ISBN 978-953-307-109-1).
Articles
International peer-reviewed journals 1. Amaral AJ, Andrade J, Foxall RB, Matoso P, Matos AM, Soares RS, Rocha CC, Ramos CG, Tendeiro R, Serra-Caetano A, Guerra-Assunção JA, Santa-Marta M, Gonçalves J, Gama-Carvalho M, Sousa AE (2017). miRNA profiling of human naive CD4 T cells links miR-34c-5p to cell activation and HIV replication. EMBO J 36 (3), 346-360. IF – 9.6; 2. Moreira AS, Sousa SA, Mil-Homens D, Pinto-de-Oliveira A, Coutinho CP, Ramos CG, dos Santos SC, Fialho AM, Leitão JH, Sá-Correia I (2017). Variation of Burkholderia cenocepacia virulence potential during cystic fibrosis chronic lung infection. Virulence 8:6, 782-796. IF – 5.418; 3. Ramos CG, Grilo AM, Sousa SA, Feliciano JR, Da Costa PJP and Leitão JH, (2014) Regulation of Hfq mRNA and protein levels in Escherichia coli and Pseudomonas aeruginosa by the Burkholderia cenocepacia MtvR. PLoS ONE 9 (6): e98813, IF – 3.234; 4. Ramos CG, Grilo AM, da Costa PJP, Leitão JH, (2013) Experimental identification of small non-coding regulatory RNAs in the opportunistic human pathogen Burkholderia cenocepacia, Genomics 101 (2): 139-148; IF – 2.801; 5. Ramos CG, Da Costa PJP, Döring G, and Leitão JH, (2012) The novel cis-encoded small RNA h2cR is a negative regulator of hfq2 in Burkholderia cenocepacia. PLoS ONE 7(10):e47896; IF – 4.079; 6. Mendes S, Farinha A, Ramos CG, Leitão JH, Viegas CA, Martins LO, (2011) Synergistic Action of Azoreductase and Laccase Leads to Maximal Decolourisation and Detoxification of Model Dye Wastewaters. Bioresource Technology 102 (21): 9852-9859; IF – 4.98; 7. Sousa SA, Ramos CG, Leitão JH. (2011) Burkholderia cepacia complex: emerging multi-host pathogens equipped with a wide range of virulence factors and determinants. International Journal of Microbiology Vol. 2011 , Article ID 607575; IF – NA; 8. Nadais H, Barbosa M, Capela I, Arroja L, Ramos CG, Grilo AM, Sousa SA, Leitão JH. (2011) Enhancing Wastewater Degradation and Biogas Production by Intermittent Operation of UASB Reactors. Energy. 36: 2164-2168; IF – 3.487; 9. Leitão JH, Sousa SA, Ferreira AS, Ramos CG, Silva IN, Moreira LM. (2010) Pathogenicity, virulence factors, and strategies to fight against Burkholderia cepacia complex pathogens and related species. Applied Microbiology and Biotechnology 87: 31-40; IF – 3.28; 10. Ramos CG, Sousa SA, Grilo AM, Eberl L, Leitão JH. (2010) The Burkholderia cenocepacia K56-2 pleiotropic regulator Pbr, is required for stress resistance and virulence. Microbial Pathogenesis 48: 168-177; IF – 2.0; 11. Sousa SA*, Ramos CG*, Moreira LM, Leitão JH. (2010) The hfq gene is required for stress resistance and full virulence of Burkholderia cepacia to the nematode Caenorhabditis elegans. Microbiology 156: 896–908. *joint first authors; IF – 2.957; 12. Ferreira AS, Leitão JH, Silva IN, Pinheiro PF, Sousa SA, Ramos CG, Moreira LM. (2010) Distribution of Cepacian Biosynthetic Genes among Environmental and Clinical Strains of the Burkholderia Genus and Role of this Exopolysaccharide on Resistance to Stress Conditions. Applied and Environmental Microbiology 76: 441-450; IF – 3.778; 13. Ramos CG, Grilo AM, Sousa SA, Barbosa ML, Nadais H, Leitão JH. (2010) A new methodology combining PCR, cloning and sequencing of clones discriminated by RFLP for the study of microbial populations: application to an UASB sample", Applied Microbiology and Biotechnology 85: 801-806; IF – 3.28; 14. Sousa SA, Ramos CG, Almeida F, Meirinhos-Soares L, Wopperer J, Schwager S, Eberl L, Leitão JH. (2008) Burkholderia cenocepacia j2315 acyl carrier protein: a potential target for antimicrobials development?, Microbial Pathogenesis 45, 331-336; IF – 2.289; 15. Ramos CG, Costa MT. (2006) Patterns of resistance to beta-lactams and beta-lactamase inhibitors in uropathogenic Escherichia coli isolated from animals in Portugal. African Journal of Biotechnology 5: 523-525. IF – 0.6.
Proceedings 1. Grilo A. M., Ramos C.G., Sousa S. A., Arroja L, Capela I., Leitão J.H., Nadais H. (2011) Optimizing energy production from wastewater by intermittent operation of anaerobic reactors. 19th European Biomass Conference and Exibition. Berlin, Alemanha 2. Grilo AM, Ramos CG, Sousa SA, Arroja LG, Capela IF, Leitao JH, Nadais HG: "Microbial population shifts as a tool for improving biogas production in anaerobic reactors", In CEST2011: A-625-629, (Sep 2011), Rhodes, Greece. 3. Ramos CG, Sousa SA, Grilo AM, da Costa PJP, Feliciano JR, Leitão JH. (2011) A RNomics-based strategy identifies regulatory small RNAs in Burkholderia cepacia complex. Proceedings of the First Portuguese Meeting in Bioengineering, 1-4 March, IST, TagusPark, Oeiras, Portugal; 4. Sousa SA, Ramos CG, Feliciano JR, Leitão JH. (2011) Identification and exploitation of Burkholderia cepacia complex virulence factors as potential antimicrobial targets. Proceedings of the First Portuguese Meeting in Bioengineering, 1-4 March, IST, TagusPark, Oeiras, Portugal; 5. Barbosa M, Ramos CG, Grilo AM, Sousa SA, Capela I, Arroja L, Leitão JH, Nadais H. (2009) Bioenergy production from complex wastewaters, Proceedings of the 5th International Conference on Energy, Environment, Ecosystems and Sustainable Development (EEESD '09), Vouliagmeni, Athens, Greece, September 28-30, 2009. Energy and Environmental Engineering Series, Published by WSEAS Press. ISSN: 1790-5095, ISBN: 978-960-474-125-0.
National peer-reviewed journals 1. Ramos CG*, Grilo AM, Sousa SA, Leitão JH*. (2013) Post-transcriptional regulation of gene expression in the human opportunistic pathogens of the Burkholderia cecepacia complex. Magazine SPM 3 (1): http://magazinespm.wordpress.com/2014/04/21/post-transcription-regulation-by-srnas-and-rna-chaperones/ * Joint corresponding authors 2. Sousa SA, Ramos CG, Grilo AM, Feliciano JR, da Costa PJP, Leitão JH. (2013), Estratégias de identificação de novos alvos para combater infecções por bactérias do complexo Burkholderia cepacia. Boletim de Biotecnologia. 2 (4): 41-43 3. Ramos CG, (2013) Roles played by Hfq-like RNA chaperones and sRNAs on the virulence of Burkholderia cepacia complex bacteria. Magazine SPM 2 (1): PhD Highlights (http://magazinespm.wordpress.com/2013/03/28/phd-highlights/); 4. Grilo AM, Ramos CG, Sousa SA, Barbosa ML, Nadais H, Leitão JH. (2010), Caracterização molecular de populações microbianas em reactores anaeróbios para o tratamento de efluentes. Boletim de Biotecnologia. 85: 3-9; 5. Ramos CG, Simões S. (2006) Métodos para a identificação de Escherichia coli na Indústria Alimentar. Revista Portuguesa de Ciencias Veterinárias 101: 131-132.
Nucleotide sequences in GenBank 1. Ramos CG, Grilo AM, Sousa SA, Nadais H, Leitão JH. Dairy wastewater treatment UASB reactor uncultured bacterium clone IST-F3 16S ribosomal RNA gene, partial sequence. Access number GQ428132; 2. Ramos CG, Grilo AM, Sousa SA, Nadais H, Leitão JH. Dairy wastewater treatment UASB reactor uncultured bacterium clone IST-C_1 16S ribosomal RNA gene, partial sequence. Access number GQ428133; 3. Ramos CG, Grilo AM, Sousa SA, Nadais H, Leitão JH. Dairy wastewater treatment UASB reactor uncultured bacterium clone IST-C_2 16S ribosomal RNA gene, partial sequence. Access number GQ4281324; 4. Ramos CG, Grilo AM, Sousa SA, Nadais H, Leitão JH. Dairy wastewater treatment UASB reactor uncultured bacterium clone IST-C_3 16S ribosomal RNA gene, partial sequence. Access number GQ428135; 5. Ramos CG, Sousa SA, Leitão JH. Nucleotide sequence of Burkholderia cenocepacia strain K56-2 locus encoding the Pbr protein required for phenazine biosynthesis. Access number EU874251; 6. Sousa SA, Ramos CG, Leitão JH. Nucleotide sequence of the Burkholderia cepacia IST408 hfq gene. Access number EU760353.
Microarray data in GEO (Genome Expression Omnibus) Ramos CG, Leitão JH, Becker JD, Moreira LM. Expression data from Burkholderia cenocepacia J2315 hfq and hfq2 mutants. Accession number GSE47344.
Oral presentations in international meetings 1. Ramos CG, Andrade J, Muniz C, Domingues N, Amaral A, Almeida S, Sousa AE, Gama-Carvalho M. microRNA 34c-5p modulates HIV-1 reactivation in latently infected T-cells. EMBO|EMBL Symposia: Innate Immunity in Host-Pathogen Interactions, June 26 – 29 2016, Heidelberg, Germany. 2. Ramos CG, Andrade J, Gama-Carvalho M. Impact of small RNA molecules derived from the 5’ ends of tRNAs in cell function. Non-coding RNA in gene regulation symposium, PS76, YSF2015-FEBS Meeting, 2-4 July 2015 Berlin, Germany. 3. Ramos CG, da Costa PJP, Grilo AM, Feliciano JR, Leitão JH. The MtvR sRNA is involved in the regulation of hfq. Microbiotec’13, 6-8 Dezembro 2013, Aveiro, Portugal 4. Ramos CG, Grilo AM, da Costa PJP, Feliciano, JR, Leitão JH. Hfq-like Towards the unveiling of the biological roles played by small non-coding RNAs in the opportunistic pathogens of the Burkholderia cepacia complex. FEBS 2012 RNA Satellite Meeting, 1-4 Setembro 2012, Tavira, Portugal; 5. Ramos CG, da Costa PJP, Grilo AM, Becker J., Moreira LM, Leitão JH. Hfq-like RNA chaperones and sRNAs: A new layer of complexity in virulence regulation. Microbiotec’11, 1-3 Dezembro 2011, Braga, Portugal; 6. Sousa SA, Ramos CG, Grilo AM, Feliciano JR, Leitão JH. Burkholderia cenocepacia J2315 hfq2 gene plays a role on stress resistance and virulence.4rd Congress of European Microbiologists (FEMS 2011), 25 - 30 June 2011. Geneva, Switzerland; 7. Ramos CG, Sousa SA, Grilo AM, da Costa PJP, Feliciano JR, Leitão JH. A RNomics-based strategy identifies regulatory small RNAs in Burkholderia cepacia complex. First Portuguese Meeting in Bioengineering, 1-4 March 2011, IST, TagusPark, Oeiras, Portugal; 8. Sousa SA, Ramos CG, Feliciano JR, Leitão JH. Identification and exploitation of Burkholderia cepacia complex virulence factors as potential antimicrobial targets. First Portuguese Meeting in Bioengineering, 1-4 March 2011, IST, TagusPark, Oeiras, Portugal; 9. Ramos CG, Sousa SA, Moreira LM, Leitão JH. The hfq gene from Burkholderia cepacia complex bacteria is required for full virulence to the nematode Caenorhabditis elegans, 3rd Congress of European Microbiologists (FEMS), June 28 – July 3 2009, Gotenborg, Sweden; 10. Sousa SA, Ramos CG, Soares L, Eberl L, Leitão JH. Burkholderia cenocepacia J2315 acp: Identification of a potential target for the development of new antimicrobials, XVIth National Congress of Biochemistry, 22-25 October 2008; Azores, Portugal; 11. Sousa SA, Ramos CG, Leitão JH. Burkholderia cenocepacia J2315 acyl carrier protein (ACP): a potential target for the development of antimicrobials against infections caused by B. species? IUMS Congresses 2008 (XII. International Congress of Bacteriology and Applied Microbiology), 5-9 August 2008; Istanbul, Turkey; 12. Sousa SA, Ramos CG, Leitão JH. The Acyl Carrier Protein, involved in the virulence of the Burkholderia cenocepacia J2315, is conserved within the Burkholderia genus. International Burkholderia cepacia Working Group Meeting 2008, 14-17 April 2008, Treviso, Italy.
Abstract in International Journals 1. Ramos CG, Andrade J, Gama-Carvalho M (2015) Impact of small RNA molecules derived from the 5’ ends of tRNAs in cell function. FEBS Journal, 282 (S1), 272. 2. Ramos C.G., Grilo A.M., da Costa P.J.P., Feliciano J.R., Leitão J.H. (2013) “Unveiling the roles of small non-coding RNAs and RNA chaperones on the biology of opportunistic pathogens of the Burkholderia cepacia complex”. Journal of Cystic Fibrosis 12, (S1):S77. 3. Sousa S.A., Soares-Castro P., Feliciano J.R., Ramos C.G., Martinez J.L.C., Santos P.M., Leitão J.H., (2013) “Unveiling the Burkholderia cenocepacia J2315 RNA chaperone Hfq2 interactome”. Journal of Cystic Fibrosis 12, (S1):S77. 4. Sousa S.A., Feliciano J.R., Ramos C.G., Leitão J.H. (2013) “The Burkholderia cenocepacia J2315 small non-coding RNA MavA is required for fitness and virulence”. Journal of Cystic Fibrosis 12, (S1):S76. 5. Pinto-de-Oliveira A., Coutinho C.P., Ramos C.G., Sousa S.A., de Carvalho C.C.C.R., Leitão J.H., Sá-Correia I. (2013) “The Burkholderia cepacia small colony variants (SCV) are a more pathogenic bacterial form that may facilitate persistent respiratory infections in CF patients”. Journal of Cystic Fibrosis 12, (S1):S76. 6. Mendes S, Farinha A, Ramos CG, Leitão JH, Viegas CA, Martins LO, (2011) Synergistic Action of Azoreductase and Laccase Leads to Maximal Decolourisation and Detoxification of Model Dye-containing Wastewaters. Environmental Engineering and Management Journal 11 (3) Supplement: S1.1 (S4) 7. Ramos CG, Sousa SA, Leitão JH. (2008), Identification and characterization of a phenazine biosythetic locus required for virulence of Burkholderia cenocepacia K56-2 to the nematode Caenorhabditis elegans, FEBS Journal, 275 (S1): 275.
Abstracts in International Meetings 1. Ramos CG, Klein AD, Futerman AH & Carmo-Fonseca M (2017) Exploring the impact of noncoding genetic variation on lysosomal function. 21st meeting of the Portuguese Society for Human Genetics, 16-18 November, Almada, Portugal 2. Ramos CG, Muniz C, Domingues N, Andrade J, Matos AM, Rocha MP, Sousa AE & Gama-Carvalho M. (2017) Rewiring NF-B mediated T-cell activation: Roles for miR-34c-5p, SINAL 2017 - 8th Meeting on Signal Transduction, 1-2 june, Lisboa, Portugal 3. Ramos CG, Andrade J, Muniz C, Domingues N, Amaral A, Almeida S, Sousa AE, Gama-Carvalho M. microRNA 34c-5p modulates HIV-1 reactivation in latently infected T-cells. EMBO|EMBL Symposia: Innate Immunity in Host-Pathogen Interactions, June 26 – 29 2016, Heidelberg, Germany. 4. Ramos CG, Andrade J, Gama-Carvalho M. Impact of small RNA molecules derived from the 5’ ends of tRNAs in cell function. Non-coding RNA in gene regulation symposium, PS76, YSF2015-FEBS Meeting, 2-4 July 2015 Berlin, Germany. 5. Ramos CG, Grilo AM, da Costa PJP, Feliciano JR, Leitão JH. The MtvR sRNA is a major regulator of Burkholderia cenocepacia cell physiology. FEBS-ASM meeting: RNA biology in host-pathogen interactions, 26-29 January, Tenerife, Spain, 2014; 6. Grilo AM, Ramos CG, da Costa PJP, Leitão JH. Identification of small non-coding RNAs and their roles on the biology of opportunistic pathogens of the Burkholderia cepacia complex. Microbiotec’13, 6-8 Dezembro, Aveiro, Portugal, 2013; 7. Ramos CG, Grilo AM, Silva IN, Ferreira AS, Becker JD, da Costa PJP, Feliciano JR, Sousa SA, Moreira LM, Leitão JH. Building an atlas of Hfq-dependent small non-coding RNAs from the opportunistic human pathogen Burkholderia cenocepacia J2315. Microbiotec’13, 6-8 Dezembro, Aveiro, Portugal, 2013; 8. Ramos CG, da Costa PJP, Grilo AM, Feliciano JR, Leitão JH. The MtvR sRNA is involved in the regulation of hfq. Microbiotec’13, 6-8 Dezembro, Aveiro, Portugal, 2013; 9. Ramos CG, Grilo AM, Feliciano JR, Leitão JH. Post-transcriptional regulation in bacterial pathogens: Small non-coding RNAs and RNA chaperones. Molecular Biology in Portugal and EMBL, 18 July, Lisbon, Portugal, 2013; 10. Ramos CG, da Costa PJP, Döring G, Leitão JH, “Hfq and sRNAs: A new layer of complexity in virulence regulation”, The 4th EMBO Meeting, 22-25 September, Nice, France, 2012; 11. Grilo AM, Ramos CG, Leitão JH, Identification of regulatory small non-coding RNAs in Burkholderia cepacia J2315. 22nd IUBMB and 37th FEBS Meeting, 4-9 September, Seville, Spain, 2012; 12. da Costa PJP, Ramos CG, Grilo AM, Leitão JH. Experimental identification of regulatory small non-coding RNAs from bacteria of the Burkholderia cepacia complex. FEBS International Workshop: New Developments in RNA Biology, 1-4 September, Tavira, Portugal, 2012; 13. Ramos CG, da Costa PJP, Leitão JH. The Burkholderia cenocepacia RNA chaperone Hfq2 is negatively regulated by the novel cis-encoded small RNA h2cR. FEBS International Workshop: New Developments in RNA Biology, 1-4 September, Tavira, Portugal, 2012; 14. Leitão JH, Capela I, Arroja L, Sousa SA, Ramos CG, Grilo AM, Nadais H. Caracterização molecular de populações microbianas de reactores anaeróbios a operar contínua ou intermitentemente para o tratamento de efluentes, IST-Ambiente - 1a Workshop sobre Ciências e Engenharia do Ambiente, IST, 22 de Novembro, 2011; 15. Grilo AM, Ramos CG, Sousa SA, Arroja L, Capela I, Leitão JH, Nadais H. Optimizing energy production from wastewater by intermittent operation of anaerobic reactors. 19th European Biomass Conference and Exibition, 6-10 June, Berlin, Germany, 2011; 16. Ramos CG, Leitão JH, The cis-encoded h2cR small non-coding RNA of Burkholderia cenocepacia J2315 is a post-transcriptional negative regulator of hfq2. MICROBIOTEC 2011, 1-3 Dezembro, Braga, Portugal, 2011; 17. Mendes S, Farinha A, Ramos CG, Leitão JH, Viegas CA, Martins LO. Synergistic action of azoreductase and laccase leads to maximal decolourisation and detoxification of model dye-containing wastewaters, MICROBIOTEC 2011, Braga 1-3 Dezembro, Portugal, 2011; 18. Grilo AM, Ramos CG, Sousa SA, Nadais H, Leitão JH. Molecular characterization of microbial populations from UASB reactors treating dairy industry wastewaters. MICROBIOTEC 2011, Braga 1-3 Dezembro, Portugal, 2011; 19. Ramos CG, Sousa SA, Grilo AM, da Costa PJP, Feliciano JR, Leitão JH. Regulating the regulator: how sRNAs keep the balance of RNA chaperones Hfq and Hfq2 in B. cenocepacia J2315. Jornadas de Engenharia Química e Biológica, IST, Lisboa, Portugal, 21-25 March 2011; 20. Ramos CG, Sousa SA, Feliciano JR, Grilo AM, Leitão JH, Functional analysis of the Burkholderia cenocepacia J2315 hfq2 gene encoding an unsual RNA chaperone, and identification of the small non-coding RNA LhdR. The Non Coding Genome EMBO-EMBL Symposium, 13-16 October , Heidelberg, Germany, 2010; 21. Ramos CG, Leitão JH. Regulatory hierarchy: Hfq orchestrates hfq2 expression in Burkholderia cenocepacia J2315. The Non Coding Genome EMBO-EMBL Symposium, 13-16 October, Heidelberg, Germany, 2010; 22. Sousa SA, Ramos CG, Leitão JH. Roles played by the RNA chaperone Hfq on the Burkholderia cepacia resistance to stress and virulence. Cellular Host-Pathogen Interactions. A Current Opinion in Cell Biology Conference, 5-7 September, Amsterdam, The Netherlands, 2010; 23. Ramos CG, Sousa SA, Feliciano JR, Vaz R, Leitão JH. Hfq sRNA chaperones: Pleiotropic regulators with crucial roles in RNA metabolism and virulence in Burkholderia cepacia complex bacteria. The EMBO meeting, 4-7 September, Barcelona, Spain, 2010; 24. Ramos CG, Sousa SA, Moreira LM, Leitão JH, The sRNA chaperone Hfq is a pleiotropic regulator required for virulence of bacteria of the Burkholderia cepacia complex. 15th Annual Meeting of the RNA Society, 22-26 June, University of Washington, Seattle, USA, 2010; 25. Ramos CG, Sousa SA, Grilo AM, Leitão JH, The Pbr transcription regulator of Burkholderia cenocepacia K56-2 is required for phenazine production and virulence. 3rd Congress of European Microbiologists (FEMS), June 28 – July 3, Gotenborg, Sweden, 2009; 26. Gil FN, Costa CP, Ramos CG, Leitão JH, Martins LO, Becker JD, Viegas CA. Exploiting S. cerevisiae and C. elegans- based eukaryotic screening bioassays to diagnose potentially adverse effects of xenobiotics in environmental samples. BioMicroWorld2009 – III International Conference on Environmental, Industrial and Applied Microbiology, December, Lisbon, Portugal, 2009; 27. Gil FN, Costa CP, Ramos CG, Leitão JH, Martins LO, Becker JD, Viegas CA. Exploiting S. cerevisiae and C. elegans- based eukaryotic screening bioassays to diagnose potentially adverse effects of xenobiotics in environmental samples. MicroBiotec09, November, Vilamoura, Portugal, 2009; 28. Barbosa M, Ramos CG, Grilo AM, Sousa SA, Capela I, Arroja L, Leitão JH, Nadais H. Bioenergy Production from Complex Wastewaters. 5th International Conference on Energy, Environment, Ecosystems and Sustainable Development (EEES'09), 28-30 September, Athens, Greece, 2009; 29. Sousa SA, Ramos CG, Moreira LM, Leitão JH, The Burkholderia cepacia complex RNA chaperone Hfq is required for stress resistance and virulence to the nematode Caenorhabditis elegans. ProkaGENOMICS - 4th European conference on Prokaryotic Genomics, 4-7 October, Göttingen, Germany, 2009; 30. Ramos CG, Grilo AM, Sousa SA, Capela I, Arroja L, Leitão JH, Nadais H. Characterization of microbial populations developed in UASB reactors operated in continuous and intermittent mode. ASPD5 Specialized Conference on Microbial Population Dynamics in Biological Wastewater Treatment, May 24-27, Aalborg, Denmark, 2009; 31. Sousa SA, Ramos CG, Costa C, Viegas CA, Moreira LM, Leitão JH. Exploiting Caenorhabditis elegans as a model system to study virulence factors and mechanisms of the Burkholderia cepacia complex and to assess xenobiotics toxicity. First IBB Meeting, May 15-16, Faro, Portugal, 2009; 32. Mil-Homens D, Ferreira AS, Sousa SA, Ramos CG, Silva IN, Oliveira S, Fialho AM, Moreira LM, Leitão JH. Virulence factors in Burkholderia cepacia complex: the contribution of cell surface structures and small non-coding RNAs. First IBB Meeting, 15-16 May, Universidade do Algarve, Faro, 2009; 33. Ramos CG, Sousa SA, Leitão JH, Identification and characterization of a phenazine biosythetic locus required for virulence of Burkholderia cenocepacia K56-2 to the nematode Caenorhabditis elegans. 33rd FEBS Congress & 11th IUBMB Conference (Biochemistry of Cell regulation), June 28 – July 3, Athens, Greece, 2008; 34. Sousa SA, Ramos CG, Grilo AM, Nadais H, Leitão JH. Molecular characterization of microbial populations in sludge bed reactors for dairy industry wastewater treatment. XVI National Congress of Biochemistry, 22-25 October, Ponta Delgada, Açores, Portugal, 2008; 35. Ferreira AS, Leitão JH, Silva IN, Sousa SA, Ramos CG, Moreira LM. Extension of the bce cluster of genes involved in the biosynthesis of cepacian by the genus Burkholderia. XVI National Congress of Biochemistry, 22-25 October, Ponta Delgada, Açores, Portugal, 2008; 36. Ramos CG, Sousa SA, Leitão JH. Identification of a phenazine biosynthetic locus necessary for full virulence of Burkholderia cenocepacia K56-2 to the nematode Caenorhabditis elegans, MICROBIOTEC XXXIII JPG 2007, 30 Novembro – 2 Dezembro, Lisboa, Portugal, 2007; 37. Sousa SA, Ramos CG, Leitão JH. Unveiling the role of Hfq on the virulence of Burkholderia cepacia complex. RNA 2007 - IV Encontro Nacional, 8-10 Novembro, Vimeiro, Portugal, 2007; 38. Ramos CG, Sousa SA, Wopperer J, Eberl L, Leitão JH. Isochorismatase plays a role on the virulence of Burkholderia cepacia in the nematode Caenorhabditis elegans. XVth National Congress of Biochemistry, 8-10 December, Aveiro, Portugal, 2006.
Online data 1. h2cR is required for B. cenocepacia J2315 virulence to C. elegans.: https://figshare.com/articles/_h2cR_is_required_for_B_cenocepacia_J2315_virulence_to_C_elegans_/227910
2. h2cR regulates hfq2 mRNA levels: https://figshare.com/articles/_h2cR_regulates_hfq2_mRNA_levels_/227608
3. h2cR is required for B. cenocepacia J2315 virulence to C. elegans: https://figshare.com/articles/_h2cR_is_required_for_B_cenocepacia_J2315_virulence_to_C_elegans_/227910
4. Analysis of the effect of h2cR overexpression or silencing on the Hfq2 protein and hfq2 mRNA: https://figshare.com/articles/_Analysis_of_the_effect_of_h2cR_overexpression_or_silencing_on_the_Hfq2_protein_and_hfq2_mRNA_/227736
5. h2cR interacts specifically with the 5′UTR of hfq2 mRNA: https://figshare.com/articles/_h2cR_interacts_specifically_with_the_5_UTR_of_hfq2_mRNA_/227801
6. The h2cR sRNA affects the levels of the Hfq2 protein and the hfq2 mRNA in B. cenocepacia cells infecting C. elegans: https://figshare.com/articles/_The_h2cR_sRNA_affects_the_levels_of_the_Hfq2_protein_and_the_hfq2_mRNA_in_B_cenocepacia_cells_infecting_C_elegans_/227971
7. Genome location and transcriptional analysis of the h2cR sRNA in B. cenocepacia J2315: https://figshare.com/articles/_Genome_location_and_transcriptional_analysis_of_the_h2cR_sRNA_in_B_cenocepacia_J2315_/227518
8. MtvR regulates the mRNA levels of the E. coli and P. aeruginosa hfq genes: https://figshare.com/articles/_MtvR_regulates_the_mRNA_levels_of_the_E_coli_and_P_aeruginosa_hfq_genes_/1048704
9. MtvR regulates hfqEc expression through interaction with its 5′-leader region: https://figshare.com/articles/_MtvR_regulates_hfq_Ec_expression_through_interaction_with_its_5_8242_leader_region_/1048708
10. MtvR affects E. coli Hfq translation: https://figshare.com/articles/_MtvR_affects_E_coli_Hfq_translation_/1048717
11. MtvR expression affects E. coli and P. aeruginosa growth kinetics and survival to prolonged nutrient deprivation: https://figshare.com/articles/_MtvR_expression_affects_E_coli_and_P_aeruginosa_growth_kinetics_and_survival_to_prolonged_nutrient_deprivation_/1048724
12. MtvR expression in E. coli and P. aeruginosa reduces biofilm formation ability and increases susceptibility to stresses: https://figshare.com/articles/_MtvR_expression_in_E_coli_and_P_aeruginosa_reduces_biofilm_formation_ability_and_increases_susceptibility_to_stresses_/1048732
13. MtvR expression in E. coli and P. aeruginosa reduces biofilm formation ability and increases susceptibility to stresses: https://figshare.com/articles/_MtvR_expression_in_E_coli_and_P_aeruginosa_reduces_biofilm_formation_ability_and_increases_susceptibility_to_stresses_/1048732
14. The MtvR sRNA also targets uhpA gene in E. coli: https://figshare.com/articles/_The_MtvR_sRNA_also_targets_uhpA_gene_in_E_coli_/1048733
15. Wikipedia entry: H2cR sRNA: Is a Negative Regulator of hfq2 in Burkholderia cenocepacia". PLoS ONE. 7 (10): e47896. doi:10.1371/journal.pone.0047896. PMC 3474761 . PMID 23082228. 786 bytes (93 words) - 07:24, 31 May 2016
