User:Charlynn Burgess/Notebook/ATP Synthesis/2011/12/01
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For the UV Vis graphs: The two graphs show a different scaling for the data in order to show the absorbance features of each sample. Cuvettes 1 and 2 were used in this data as indicated. Each data set is corrected to remove the absorbance contributions of the solvent (water).
For the fluorescence graphs: For each sample a 450nm excitation beam was used and the fluorescence was recorded from 500nm to 700nm. Each spectrum has been corrected to remove the contributions of the solvent (water). The two graphs are separated due to different experimental conditions. Graph 1 used 10nm excitation and emission slits and ran at a speed of 500nm/min. Graph 2 used 5nm excitation and emission slits and ran at a speed of 60nm/min. The names of the spectra shown here correspond to the UV vis spectra taken in the fluorescence cuvette as shown in the figures above.