Entry title
Objective
- To find the sensitivity of the fluorometer using Ru(bpy)(phen) in acetonitrile.
Data
First, standards of Ru(bpy)(phen) in acetonitrile were made. The stock solution of Ru(bpy)(phen) is 360μM:
Standard |
Amount of Ru(bpy)(phen) |
Amount of acetonitrile |
Final Concentration in Cuvette
|
Standard 1
|
60 μL |
2940 μL |
7.2 μM
|
Standard 2
|
120 μL |
2880 μL |
14.4μM
|
Standard 3
|
180μL |
2820μL |
21.6μM
|
Standard 4
|
240μL |
2760μL |
28.8μM
|
Standard 5
|
300μL |
2700μL |
36μM
|
Standard 6
|
600μL |
2400μL |
72μM
|
Standard 7
|
900μL |
2100μL |
108μM
|
The intensities taken were corrected with the intensities of acetonitrile, and the spectrum of intensities was graphed:
The curves were integrated in Excel and the following areas under the curves were found:
Concentration (μM) |
Integrated Intensity
|
7.2
|
682.1927
|
14.4
|
886.9565
|
21.6
|
1091.243
|
28.8
|
1339.258
|
36
|
1436.954
|
72
|
1767.737
|
108
|
1877.2334
|
These integrated intensity were plotted against the concentrations to see the sensitivity of the instrument:
The calculated integrated intensities for all data collected from the class was graphed in the same way:
Notes
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