User:Cassandra M Barrett/Notebook/Open Chromatin/2015/11/13

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LCR pJET Miniprep and Verification

Purpose: to verify if Gal4DB_mCh and ATF2 were successfully assembled in the pJET vector

Methods:

Use the plasmid Miniprep kit to isolate plasmid DNA from all five colonies grown in liquid broth. Streak spots onto LB+AMP plates from each culture before miniprep. No primers in lab for pJET vector to verify insert. Will do PCR when they arrive along with possible sequencing.



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