User:Cassandra M Barrett/Notebook/Open Chromatin/2015/09/12

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Gel Verification of LCR Parts

Purpose: to verify lengths of nucleotide sequences for LCR

Methods:

Oligo bridges (diluted for LCR) and stocks for PCR of LCR parts run on 1% agrose gel for 35min at 110V to verify identity before amplification and reattempting LCR. (2uL sample and 4uL dye)

Results:

Image:LCRparts.jpg

The image shows proper length of starting plasmids and small vague bands as expected for the oligo bridges. We will proceed with part amplification via PCR next time.

B1: LCRb_ATF2 to MV10 B2: LCRb_MV10 to Gal4DB_mCh B3: LCRb_mCh to ATF2



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