User:Cassandra M Barrett/Notebook/Open Chromatin/2015/09/09
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LCR PCR Colony Screen
Purpose: To screen for proper insertion of Gal4DB_mCh and ATF2 into the MV10 vector.
GoTaq Colony PCR (1RXN):
Controls: - control of water only, MV10
6 colonies selected from LCR1 and 6 colonies from LCR2 Incubated in 10uL of mastermix for 10min at RT Run on GoTaq PCR program (60C, 30min)