User:Cassandra M Barrett/Notebook/Haynes Lab/2015/09/07

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Transfection with Rene

Purpose: to learn about basic lipofectamine ltx transfection

Methods:

4 plasmids- find their concentrations first, calculate how many uL to get 2ug of DNA

9057 in pX330g mp: 219 ng/uL --> 9uL 9058 in pX330g mp: 243 ng/uL --> 8uL 9059 in pX330g mp: 185 ng/uL --> 11uL 9060 in pX330g mp: 253 ng/uL --> 8uL

Refer to the following protocol: http://openwetware.org/wiki/Haynes:TransfectionPlasmid_Lipo



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